Coding

Part:BBa_K2818001:Design

Designed by: Danny Teo Shun Xiang   Group: iGEM18_NTU-Singapore   (2018-10-07)


Cas13d-NLS-ADAR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2935
    Illegal BamHI site found at 2965
    Illegal XhoI site found at 2410
    Illegal XhoI site found at 3666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NLS is found to improve RNA editing.


Source

Ruminococcus flavefaciens

References